Ther. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Physicochemical studies of oligodextran. The term f/f0 is sometimes denoted as P, Perrin constant. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). International Journal of Pharmaceutics, 277, 99-104. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. De Belder, A.N. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Kasaai, Mohammad R. (2007). (2004). Molecular weight (M v) and hydrodynamic properties can be calculated from there. The receptor chamber was filled with PBS. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Garcia de la Torre, J.; Carrasco, B. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly
Nanoparticles in magnetic resonance imaging: from simple to dual The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 The human and pig skin permeability of FITC-dextran (M.W. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Chem. 2018;1:10915. WebR H Hydrodynamic radius [m] The basic DLS setup. Weissella: An Emerging Bacterium with Promising Health Benefits. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). The authors declare that they have no competing interests. 7 8 Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. The above data are not useful because it can not be compared with those obtained in this work. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Elias, Hans-Georg (1975). If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Die Makromolekulare Chemie, 148(1), 229-238.
dextran hydrodynamic radius European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Dynamics of a Polymer Solution in a Rigid Matriz. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). ; Veldhuis, T.F.J. (1987). 4K and M.W. 2011;5(2):95132.
Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Advances in Cosmetic Surgery. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Webdextran hydrodynamic radius. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Polysaccharide hydrogels for modified release formulations. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. On single point determination of intrinsic viscosity. Macromolecules, 33, 5730-5739. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). J Control Release. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Pathan IB, Setty CM. Carbohydrate Polymers 88 1440-1444. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. 2011;16(2):518. Format), Citation-(EndNote
Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0.
Structural and biocompatibility properties of dextran from Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. J. Braz.
Polymers | Free Full-Text | Confinement-Induced Fractionation and Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Nair SS. 2012;29(7):201729. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle.
dextran 2013;3(5):3588605. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Alternatives to animal testing: a review.
Molecular dynamics simulations on aqueous There is a deviation from linearity after 580kDa [69, 70]. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections.
Hydrodynamic Radius Learn More. Sung-Wook Choi. Dextran. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. FITC-dextran solution (600L) was added to the donor chamber. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. (2001). The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). This conjecture found to be correct. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Walkenhorst, S.; Olivier, R. (2002). (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. (1999). Webwhittier union high school district superintendent. WebTry out PMC Labs and tell us what you think. European. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. HHS Vulnerability Disclosure, Help An undergraduate physical chemistry experiment. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. In addition, the permeation rate was higher in pig skin than in human skin. Dextran produced from Weissella cibaria JAG8 was purified and characterized. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Journal of Physical Chemistry. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. High Manufacturing Standards of Molecular Probes Dextrans. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Webdextran hydrodynamic radius. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Hydrodynamic radii ( RH) were determined by dynamic light scattering. @ Nilimesh See attached. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. ; Patel, T.R. Masuelli, Martin Alberto (2013).
Hydrodynamic The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. This site needs JavaScript to work properly. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Sachdeva V, Banga AK. 2012;101(8):28619. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Int J Pharm Investig. Rheology of dextran solutions. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Hydrodynamic Properties of Tragacanthin. Treatment of acne scars by fractional bipolar radiofrequency energy. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Terms and Conditions, Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Proc of SPIE.
dextran Epub 2022 Feb 2. eCollection 2018 Feb. Molecules. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. (2013). The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. (2013). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Progress in Polymer Science, 30, 38-70. Drug Deliv. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. 4h after microporation, the sizes of the micropore decreased slightly. Huggins equation [41] gives the relation to the relative viscosity increase (i). Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Format). Molecular weight, molecular weight distribution and molecular size of a native dextran. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Increased TEWL values are evidence of micropores formation. Nat Biotechnol. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). Dependence on Concentration.
Protein Hydrodynamics with Light Scattering Intrinsic viscosity-molecular weight relationship for chitosan. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Data File Dextran. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Ind. 2019;26(1):25261. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Advances in chemistry series Washington, DC: American Chemical Society., pp. The Viscosity of Dilute Solutions of Long-Chain Molecules. Correspondence to WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. (2003). Front Microbiol.
Hydrodynamic Radiofrequency with microneedling.
Dextran NCI CPTC Antibody Characterization Program. (2006). Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Carbohydrate Research, 160, 205-214. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). (2003). ; Prasad, D.H.L. (1954). Viscosity of Liquids. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Microporation applications for enhancing drug delivery.
The Hydrodynamic Radii of Macromolecules and Their (1990). Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Viswanath, D.S. Herwadkar A, Banga AK. Dilute Solution. Journal of American Chemical Society, 76 (20), 5041-5052. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. One day before the experiment, the skin was stabilized in the skin culture medium. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. dextran hydrodynamic radius. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Microporation was performed 2, 5, and 10 times on each sample. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Analytical Chemistry. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Journal Argentine Chemical Society 100 (2013) 25-34. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Solution properties of branched dextran. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the